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. 2012 Nov 30;7(11):e50722. doi: 10.1371/journal.pone.0050722

Figure 1. Langerin ECD interaction onto heparin.

Figure 1

Surface was functionalized with heparin 6 kDa. 100 µL Langerin ECD at 500 nM are injected onto the surface in a Ca2+ containing running buffer. Two modes of surface regenerations are tested, 1: Injection of 30 µL of 50 mM EDTA. 2: Injection of 50 µL of 350 mM MgCl2.