Table 1. Disaccharide analysis of GAGs.

Disaccharide	Hp	Hp de6S	CS-A	CS-B	CS-C	Disaccharide
ΔHexA - GlcNAc	5.2	4.6	11	9.5	4.3	ΔHexA - GalNAc
ΔHexA - GlcNAc,6S	4.1	3.9	46.8	78.6	24.2	ΔHexA - GalNAc,4S
ΔHexA - GlcNS	6.1	10.1	41	6	52.5	ΔHexA - GalNAc,6S
ΔHexA - GlcNS,6S	12.6	9.8	0	1.2	0	ΔHexA,2S - GalNAc,4S
ΔHexA,2S - GlcNS	9.4	57.4	0.5	4.3	2.5	ΔHexA - GalNAc,4S,6S
ΔHexA,2S - GlcNS,6S	60.7	11.7	0.6	0.4	16.5	ΔHexA,2S - GalNAc,6S
ΔHexA,2S - GlcNAc	1.9	2.5
Sulfate/dp2	2.4	1.9	0.9	1	1.1	Sulfate/dp2
N-sulfation	88.8	89	0.6	1.7	16.5	2-O-sulfation
2-O-sulfation	72	71.6	47.3	84.1	26.7	4-O-sulfation
6-O-sulfation	77.4	25.4	42.1	10.7	71.5	6-O-sulfation

For determination of GAG composition, heparin and CS samples were exhaustively depolymerised (with heparinases I, II, III and chondroitinase ABC, respectively), and the resulting disaccharides were resolved by SAX-HPLC, using a NaCl gradient calibrated with authentic standards.