Figure 4. Expression of NK receptors by HLA-E-restricted CD8 T cells and functional characterization.
A/Surface expression of NK receptors by HLA-E-restricted CD8 T cells. RFI of stained T cells (thick line) are indicated. B/Modulation of HLA-E restricted CD8 T cells reactivity through NKR engagement. 51Cr-labeled P815 cells were preincubated with the indicated concentration of anti-CD3 antibody in the presence or not of the indicated anti-NKR antibody for 1 h. Then, MART.22 T cells were added for 4 h. Redirected cytotoxic activity was assessed through measure of Chromium release in the supernatants. Percentages of specific lysis are indicated. Means and standard deviations of triplicate wells are shown for one representative experiments out of three performed.