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. 2012 Nov 30;7(11):e50951. doi: 10.1371/journal.pone.0050951

Figure 6. Regulation of HLA-E-restricted CD8 T cells reactivity against allogeneic endothelial cells by NK receptor.

Figure 6

A/Reactivity of HLA-E-restricted T cells against poorly recognized (HAEC#116) or unrecognized (HAEC#402) endothelial cultures pulsed with synthetic peptides. HAECs were incubated for 1 h with range concentrations of the indicated peptides before MART.22 T cells were added. After 6 h, T cells were fixed, permeabilized and stained for intracellular TNF-α. Results are expressed as percentage of TNF-producing T cells. B/Impact of KIR2DL2-ligands expression by HAECs on HLA-E-restricted T cells alloreactivity. Percentages of TNF-producing MART.22 T cells are shown for HAECs with none, one or two protective HLA-C alleles. C/Reactivity of HLA-E-restricted T cells against unrecognized endothelial cultures (HAEC#402) in the presence of blocking antibodies. HAECs were incubated with MART.22 T cells in the presence or not of indicated concentrations of blocking antobodies. After 6 h, T cells were fixed, permeabilized and stained for intracellular TNF-α. Results are expressed as percentage of TNF-producing T cells.