Figure 6. Downregulation of COX-2 by miR-16 increases apoptosis in HCC cells.

Hep3B cells were transfected with 50 nM miR-16 or In-miR-16 in the presence or absence of 5 µM PGE₂ (A) Apoptosis was measured with Annexin V-FITC Apoptosis Detection Kit (B) Western blot analysis of caspase-3. Results are the means ± SD of three different experiments. *p< 0.05 vs. the corresponding control cells ^# p< 0.05 vs. miR-16 condition.