Figure 6. Effects of active immunization against hCG on tumor-associated pituitary transcripts.

(A) Semi-quantitative RT-PCR analysis of transcripts of tumor-associated proteins (HMGA2, E2F1 CCND1, PRL, GH, GAL, PTTG1, BMP4) and (B) CDK inhibitors (CDKN1B, CDKN2A and CDKN2C) in the pituitaries derived from TG mice, TG mice immunized with IFA (“Control TG”), TG mice immunized with hCG + IFA (“Immunized TG”), WT mice, WT mice immunized with IFA (“Control WT”) and WT immunized with hCG + IFA (“Immunized WT”). Each lane represents an individual animal. ACTB (β-actin) served as control.