Figure 6. Inhibition of NA-induced SRF activation by silodosin.

Prostate tissue from each patient was allocated to three samples, which were stimulated with NA (30 µM) for 15 min, or remained unstimulated. Silodosin (3 µM) or solvent (3 µl DMSO) were added 15 min before NA as indicated. All samples were exposed for identical total periods to experimental conditions to prevent agonist-unspecific effects. In each experiment, SRF in NA-stimulated samples without silodosin ( = DMSO) was set to 100%, and values for samples with silodosin were referred to that sample. Shown are representative experiments, and densitometric quantification of all experiments (n = 6 patients; means±SEM).