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. 2012 Nov 30;7(11):e50343. doi: 10.1371/journal.pone.0050343

Table 4. PCR reaction used in this study.

Region Nested PCR Reaction program
5′NCR/C 1st PCR 50°C for 30 min, 94°C for 1 min, 3 PCR cycles of 94°C for 1 min, 50°C for 1 min, and 72°C for 2 min, 32 PCR cycles of 94°C for 15 sec, 50°C for 30 sec, and 72°C for 1.5 min; with a final extension at 72°C for 10 min.
2nd PCR 94°C for 1 min, 3 PCR cycles of 94°C for 1 min, 55°C for 1 min,and 72°C for 1.5 min, 32 PCR cycles of 94°C for 15 sec, 55°C for 30 sec, and 72°C for 50 sec, with a final extension at 72°C for 10 min.
C/E2 1st PCR 50°C for 30 min, 94°C for 1 min, 3 PCR cycles of 94°C for 1 min, 50°C for 1min, and 72°C for 2 min; 32 PCR cycles of 94°C for 15 sec, 50°C for 30 sec, and 72°C for 1.5min, with a final extension at 72°C for 10min.
2nd PCR 94°C for 1 min, 3 PCR cycles of 94°C for 1 min, 55°C for 1 min, and 72°C for 2 min; 32 PCR cycles of 94°C for 15 sec, 55°C for 30 sec, and 72°C for 80 sec; with a final extension at 72°C for 10 min.
NS5B 1st PCR 50°C for 30 min, 94°C for 1 min, 3 PCR cycles of 94°C for 1 min, 50°C for 1 min, and 72°C for 2 min; 32 PCR cycles of 94°C for 15 sec, 50°C for 30 sec, and 72°C for 1.5 min; with a final extension at 72°C for 10 min.
2nd PCR 94°C for 1 min; 3 PCR cycles of 94°C for 1 min, 55°C for 1 min, and 72°C for 2 min; 32 PCR cycles of 94°C for 15 sec, 55°C for 30 sec, and 72°C for 80 sec; with a final extension at 72°C for 10 min.