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. 2012 Nov 30;7(11):e50987. doi: 10.1371/journal.pone.0050987

Figure 5. hp0197 controls CcpA activity by HPr-Ser-46P.

Figure 5

The purified FLAG-tagged HPr from the log-phase WT or Δhp0197 bacteria were compared to evaluate the binding of CcpA to its target site (cre) by EMSA. The HPr from the stable-phase WT strain served as a control. Significant more DNA (indicated by *) with CcpA and HPr from Log-phase WT migrated slower than DNA with CcpA and HPr from Δhp0197 or from the stable-phase WT strain, indicating that HPr from log-phase WT could enhance the binding of CcpA to cre and the HPr from log-phase Δhp0197could not enhance the ability significantly.