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. 2012 Nov 30;7(11):e50975. doi: 10.1371/journal.pone.0050975

Figure 4. Upregulation of MKP1 and MKP3 protein levels in cells treated with MG132.

Figure 4

a) Immunoblot results indicating the effect of MG132 pretreatment time on MKP1 and MKP3 upregulation in unstimulated or PDGF-stimulated NIH 3T3 cells. Total ERK1/2 levels are not significantly perturbed by MG132 treatment. The 18 bands for each antigen are cropped from the same gel and rearranged. b) Quantification of relative MKP1 and MKP3 expression levels as a function of PDGF (1 nM) stimulation time, comparing cells pretreated for 6 h with 25 µM MG132 or DMSO vehicle only. Each readout is normalized by total ERK and expressed as mean ± s.e.m. (n = 3, independent experiments). The indicated p value for each time course is from two-way ANOVA analysis comparing MG132-treated and control measurements.