Figure 4. Loss of Grb2 does not impair podocyte development in Nephrin-Cre; Grb2^flx/flx animals.

(A) Nephrin-Cre; Grb2^flx/flx animals, injected with adriamycin or saline, display a normal renal histology 9 weeks after treatment, as visualized by periodic acid–Schiff (PAS)-stained paraffin kidney sections. (B) Representative examples of ultra-structural electron microscopy analysis of kidneys of Nephrin-Cre; Grb2^flx/flx animals, injected with adriamycin or saline, compared to control (Cre-negative) littermates, 9 weeks after treatment. Bar is 500 nm.