Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Mar;35(3):1018–1023. doi: 10.1128/iai.35.3.1018-1023.1982

Mutant of Bordetella pertussis which lacks ability to produce filamentous hemagglutinin.

M Watanabe, Y Nakase
PMCID: PMC351149  PMID: 6279510

Abstract

Mutant cultures that lacked the ability to produce filamentous hemagglutinin were obtained by UV irradiation of Bordetella pertussis strain 18-323. Various biological activities of these mutants were compared with those of the parent culture. It was found that the leukocytosis-promoting, histamine-sensitizing, and mouse-protecting activities of the mutants were similar to those of the parent strain but that the virulence to mice was lower. The results seemed to rule out fimbrial hemagglutinin as a protective antigen when the ability to protect mice was measured by the intracerebral challenge test.

Full text

PDF
1018

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arai H., Sato Y. Separation and characterization of two distinct hemagglutinins contained in purified leukocytosis-promoting factor from Bordetella pertussis. Biochim Biophys Acta. 1976 Oct 22;444(3):765–782. doi: 10.1016/0304-4165(76)90323-8. [DOI] [PubMed] [Google Scholar]
  2. ELDERING G., HORNBECK C., BAKER J. Serological study of Bordetella pertussis and related species. J Bacteriol. 1957 Aug;74(2):133–136. doi: 10.1128/jb.74.2.133-136.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. KASUGA T., NAKASE Y., UKISHIMA K., TAKATSU K. Studies on Haemophilis pertussis. III. Some properties of each phase of H. pertussis. Kitasato Arch Exp Med. 1954 Sep;27(3):37–47. [PubMed] [Google Scholar]
  4. KASUGA T., NAKASE Y., UKISHIMA K., TAKATSU K. Studies on Haemophilus pertussis. I. Antigen structure of H. pertussis and its phases. Kitasato Arch Exp Med. 1953 Nov;26(2-3):121–133. [PubMed] [Google Scholar]
  5. KASUGA T., NAKASE Y., UKISHIMA K., TAKATSU K. Studies on Haemophilus pertussis. II. Resistance of each antigen to heat, alcohol and HCI. Kitasato Arch Exp Med. 1953 Nov;26(2-3):135–141. [PubMed] [Google Scholar]
  6. Kendrick P. L., Eldering G., Dixon M. K., Misner J. Mouse Protection Tests in the Study of Pertussis Vaccine: A Comparative Series Using the Intracerebral Route for Challenge. Am J Public Health Nations Health. 1947 Jul;37(7):803–810. [PMC free article] [PubMed] [Google Scholar]
  7. MASRY F. L. G. Production, extraction and purification of haemagglutinin of Haemophilus pertussis. J Gen Microbiol. 1952 Nov;7(3-4):201–210. doi: 10.1099/00221287-7-3-4-201. [DOI] [PubMed] [Google Scholar]
  8. Munoz J. J., Arai H., Cole R. L. Mouse-protecting and histamine-sensitizing activities of pertussigen and fimbrial hemagglutinin from Bordetella pertussis. Infect Immun. 1981 Apr;32(1):243–250. doi: 10.1128/iai.32.1.243-250.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nakase Y., Takatsu K., Kasuga T. Antigenic structure and phase variation in Bordetella pertussis. Jpn J Microbiol. 1969 Sep;13(3):283–291. doi: 10.1111/j.1348-0421.1969.tb00468.x. [DOI] [PubMed] [Google Scholar]
  10. PILLEMER L. Adsorption of protective antigen of Hemophilus pertussis on human red cell stromata. Proc Soc Exp Biol Med. 1950 Dec;75(3):704–705. doi: 10.3181/00379727-75-18311. [DOI] [PubMed] [Google Scholar]
  11. STANDFAST A. F. B. The phase I of Haemophilus pertussis. J Gen Microbiol. 1951 Aug;5(3):531–545. doi: 10.1099/00221287-5-3-531. [DOI] [PubMed] [Google Scholar]
  12. Sato Y., Arai H., Suzuki K. Leukocytosis-promoting factor of Bordetella pertussis. 3. Its identity with protective antigen. Infect Immun. 1974 May;9(5):801–810. doi: 10.1128/iai.9.5.801-810.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. THIELE E. H. Studies on the hemagglutinin of Hemophilus pertussis. J Immunol. 1950 Dec;65(6):627–632. [PubMed] [Google Scholar]
  14. Worcester J., Wilson E. B. A Table Determining L.D.50 or the Fifty Per Cent End-Point. Proc Natl Acad Sci U S A. 1943 Jul;29(7):207–212. doi: 10.1073/pnas.29.7.207. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES