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. 2012 Nov 30;7(11):e50399. doi: 10.1371/journal.pone.0050399

Figure 3. Procoagulant activity of DNA- and RNA-oligonucleotides with different length and secondary structures.

Figure 3

Increasing concentrations of (A) DNA-oligonucleotides 21mer-H1 (closed circles) or 21mer-L1 (closed squares) as well as (B) the respective RNA-oligonucleotides, 21mer-H1 (closed circles) or 21mer-L1 (closed squares) were tested for procoagulant activity in a turbidity clot-lysis assay using pooled human plasma. Coagulation was initiated by recalcification; clotting times were defined as respective time points of maximal absorbance. The clotting time of untreated plasma was defined as 100%. The activation of prekallikrein was followed in the presence of increasing doses of (C) DNA-oligonucleotides 21mer-H1 (closed circles) or 21mer-L1 (closed squares) as well as (D) the respective RNA-oligonucleotides 21mer-H1 (closed circles) or 21mer-L1 (closed squares). Enzyme activity was registered by chromogenic substrate assay as described in “Material and Methods”. All data represent mean ± SEM (n≥3; *p<0.05; 21mer-H1 vs. 21mer-L1).