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. 2012 Nov 30;7(11):e50547. doi: 10.1371/journal.pone.0050547

Figure 4. Temporal pattern of WA-induced BiP, GRP94, HSP30 and HSP70 accumulation.

Figure 4

Cells were exposed to 5 µM WA (A, B) or 30 µM MG132 (C, D) for time intervals ranging from 2 to 24 h. Cells were harvested and total protein was isolated. The different protein samples were then analyzed by immunoblot analysis. Image J software was used to perform densitometric analysis of the signal intensity for BiP (white), GRP94 (black), HSP30 (white) and HSP70 (black) protein bands of western blot images as described in Materials and methods and in the legend of Figure 3. The data are expressed for each treatment as a ratio to control levels for BiP and GRP94 accumulation (panels A & C) or as a percentage of the maximum band for HSP30 and HSP70 accumulation (panels B & D). Significant differences between the control cells and WA or MG132 treated cells are indicated as * (p<0.05) or Δ (p<0.10). These data are representative of three separate experiments.