Figure 1. Disease characteristics of the recurrent TNBS model and efficacy of budesonide treatment.

The development of colitis induction is evident from periods of loss of body weight following each rectal instillation of TNBS (Figure 1A; AUC p<0.005). At end-point, inflamed colons show significantly increased weight/length ratios and colon thickness (Figure 1B); both aspects are suppressed by budesonide. TNBS colitis induction is associated with a significantly increased histological score (Figure 1C); this score is significantly lower in budesonide treated animals. Bars represent group mean values of 10–12 mice/group, error bars represent SEM. Mann-Whitney U-test; *p<0.05, **p<0.01, ***p<0.001.