Figure 2. Colitis induction is associated with increased numbers of innate and adaptive immune cells.

The composition of the cellular infiltrate and the effect of budesonide treatment were analyzed by immunohistochemistry. (A) Enumeration of the cells showed significantly increased cell numbers per mm² tissue due to TNBS colitis induction. Budesonide treatment significantly reduced the number of these infiltrating cells. Bars represent group mean values of 10–12 mice/group, error bars represent SEM. Mann-Whitney U-test; *p<0.05, **p<0.01, ***p<0.001. (B) Double staining of CD11b+ and F480+ cells using fluorescence microscopy confirmed co-expression of these two cell surface markers.