Figure 4. Nuclear annexin A2 is not associated with S100A10.

(A) TIME cells were either not treated (NT) or treated with 10 Gy IR for 2 hours; (B) MCF7 cells were treated with 10 Gy IR for 2 hours; (C) A549 cells were either not treated (NT) or treated with 1 mM H₂O₂ or 10 Gy IR for 2 hours as indicated. (A–C) Nuclear and non-nuclear fractions were prepared followed by immunoprecipitation with the antibodies indicated. Nuclear and non-nuclear fractions (cell extracts) and immunoprecipitates were subjected to SDS-PAGE and analyzed by western blotting with the antibodies indicated. (D) TIME cells were either not treated (NT) or treated with 0.5 mM H₂O₂ for 30 minutes or 10 Gy IR for 1 hour. Cells were subjected to immunocytochemistry analysis with the antibodies indicated and visualized by confocal microscopy. Scale bar is 20 µM.