Table 2. Calculated EMAST mutations rates for D8S321 clones with knockdown of hMSH3.
| D8S321 (AAAG)12clone 1 | D8S321 (AAAG)12clone 2 | |
| Parental | 0×10−4±0×10−4 | 1.2×10−4±0.62×10−4 |
| Scrambled shRNA | 0×10−4±0×10−4 | 1.2×10−4±0.57×10−4 |
| hMSH3 shRNA | 18.4×10−4±0.18×10−4 a | 12.7×10−4±1.14×10−4a |
Data from the EGFP-positive population at weeks 4, 5 and 6 time points were used for -4 bp frameshift mutation rate analysis. Single mutation rates were calculated by combining and averaging time-specific mutation rates. Rates are expressed as mutations at microsatellite sequence per cell per generation. Data shown are mean±SEM.
a
represents significant difference in the mutation rate (P<0.05). hMSH3 shRNA vs. scramble shRNA (clone 1 P = 0.000082, clone 2 P = 0.003086).