Figure 6. Circadian regulation of GCL enzymatic activity.

(A) Daily profile of GCL activity in heads of CS flies as measured by the formation of the GCL product, γ-GC. Data represents average values ± SEM obtained from 4 independent bio-replicates (total N = 16). An asterisk indicates a significant difference between the peak and trough time points calculated by 1-way ANOVA and Bonferroni post-tests. (B) GCL activity was altered in per⁰¹ and cyc⁰¹ mutants such that no statistical difference was detected between time points where control CS flies showed peak at (ZT 0) and trough (ZT 8). Bars show average values ± SEM obtained from 4–5 independent bio-replicates (total N = 16). Data in (B) are analyzed by 2-way ANOVA and Bonferroni's post-tests. Different subscript letters indicate significant differences between treatment groups (p<0.05).