Figure 2. The coulter counter assay is used to measure specific homotypic cell-cell adhesion rates for HCT-8 E and R cells.

(a) Comparison of cell-cell adhesion rates of original HCT-8 E cells (never exposed to 21 kPa PA gels), disassociated HCT-8 R cells harvested from 21 kPa PA gels, and normal non-cancerous epithelial Ma104 cells. HCT8 R cells have the lowest cell-cell adhesion. Each data point consists of 3 duplicates, and each duplicate consists of 5×10⁵ cells of respective cell types.