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Level of mRNA for the subunit A of V-ATPase in hypocotyl segments. Aliquots (20 μg) of the total RNA fractions from the hypocotyl segments were subjected to northern analysis with a 32P-labeled cDNA fragment of the V-ATPase subunit A. A, Ethidium bromide staining of rRNAs. B, Northern analysis. C and D, Relative amount of mRNA for the V-ATPase subunit A on the basis of RNA or DNA content, respectively. Values shown are relative to that of segment no. 31.
