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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Nat Cell Biol. 2012 Nov 25;14(12):1336–1343. doi: 10.1038/ncb2622

Figure 5. MCUR1 is required for maintenance of cellular bioenergetics.

Figure 5

(a) AMP/ATP ratios in stable HeLa cell lines stably expressing negative shRNA, MCUR1 shRNA (clone shHe2) or ShHe2 with MCUR1 re-expressed. **P < 0.01 (mean ± s.e.m.; n=3). (b) O2 consumption rates (OCR) in stable HeLa cells expressing irrelevant shRNA, clone ShHe2, and clone ShHe2 re-expressing MCUR1, exposed sequentially to (a) oligomycin, (b) FCCP, and (c) rotenone plus myzothiazol. (c) Basal and maximal OCR in cells as described in (B). *P < 0.05 (mean ± s.e.m.; n=3). (d) Western blot of phosphorylated and total AMPK (top) and densitometric analysis (bottom) in stable HeLa lines expressing negative shRNA or MCUR1 shRNA (clone sheHe2) and clone shHe2 re-expressing MCUR1. *P < 0.05, **P < 0.01 (mean ± s.e.m.; n=3). (e) Western blot of LC3 or tubulin in stable HeLa lines expressing negative shRNA or MCUR1 shRNA (clone sheHe2) and clone ShHe2 re-expressing MCUR1 (top) and quantification of LC3-II/(LC3-I + LC3-II) (bottom) expressed as fold increase over levels in cells expressing irrelevant shRNA. *P < 0.05, **P < 0.01 (mean ± s.e.m.; n=3). (f and g), as in (d and e). Activation of AMPK (f) and autophagy (**P < 0.001; mean ± s.e.m.; n=3) (g) in absence and presence of InsP3R inhibitor Xestospongin B (XeB). *P < 0.05, **P < 0.001, ns = not significant (mean ± s.e.m.; n=3).