Alignment of Fe and Zn XRF arising from PEG-embedded samples of myocardium. (a)–(c), FA; (d)–(f) control. (a) and (d), PEG 1450 block containing heart tissue from the LVW; (b) and (e) Fe XRF; (c) and (f) Zn XRF. The Fe signal of the FA heart shows a somewhat irregular distribution with peak XRF arising from a papillary muscle (circle in b). The same region (c, circle) shows a low Zn signal. The distribution of Zn in the LVW of FA (c) is more homogeneous than that of Fe. The circles in (e) and (f) show an example of regional comparison of Fe and Zn concentrations. Bars, 5 mm.
Abbrev.: FA, Friedreich's ataxia; Fe, iron; LVW, left ventricular wall; PEG, polyethyleneglycol; XRF, X-ray fluorescence; Zn, zinc