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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Am J Cardiol. 2012 Sep 21;110(12):1820–1827. doi: 10.1016/j.amjcard.2012.08.018

Figure 2.

Figure 2

Comparison of Fe XRF maps, Fe histochemistry, ferritin and ferroportin immunohistochemistry in FA. (a)–(e), Fe XRF; (f)–(j), Fe histochemistry (brazilin counterstain); (k)–(o), ferritin immunohistochemistry; (p)–(t), ferroportin immunohistochemistry. From top to bottom, the cardiac tissues are: LVW (a, f, k, p); RVW (b, g, l, q); VS (c, h, m, r); RA (working myocardium) (d, i, n, s); AS (working myocardium) (e, j, o, t). The contiguous microphotographs correspond to the circled region on the XRF maps. All cardiomyocytes with blue Fe reaction product display abundant immunohistochemical reaction product of ferritin. Not all ferritin-positive fibers show histochemical reaction product of Fe. Ferroportin reaction product is abundant in all samples of myocardium and does not seem to change in step with the accumulation of Fe or ferritin (however, see fig. 3). Bars in Fe XRF maps, 5 mm; all other bars, 50 μm. Abbrev.: AS, atrial septum; FA, Friedreich's ataxia; Fe, iron; LVW, left ventricular wall; RA, right atrium; RVW, right ventricular wall; VS, ventricular septum; XRF, X-ray fluorescence.