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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Invest Dermatol. 2012 Aug 30;133(2):518–527. doi: 10.1038/jid.2012.317

Figure 1. Effects of ASC expression in melanoma cells.

Figure 1

(a) Representative staining of human melanoma tumors with ASC. Bar=100 μm. Arrows depict melanoma cells. (b) Western blot of ASC in human melanoma cell lines. β-Actin served as a loading control. (c) qRT-PCR of ASC after transfection with pCMV-GFP-V empty vector (pCMV-V) or pCMV-GFP-ASC vector (pCMV-ASC). Expression levels normalized to GAPDH. (d) Western blot of cells transfected with pCMV-V or pCMV-ASC. Anti-ASC antibody detects both transfected (depicted as GFP-ASC) and endogenous ASC. β-Actin served as a loading control. (e) Cell viability in 1205Lu and HS294T transfected with pCMV-V or pCMV-ASC. (f) Cell viability in WM35 and WM115 after 48 hours of transfection with pCMV-V or pCMV-ASC. Data represent mean + S.E. (n=3). ***P≤0.001 compared with transfection with pCMV-V.