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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Thromb Haemost. 2012 Sep 26;108(6):10.1160/TH12-06-0436. doi: 10.1160/TH12-06-0436

Figure 1.

Figure 1

Time course analysis of wild-type and mutant FIX activation by the TF-FVIIa complex. A, the time course of activation of FIX (◯) and FIX-desEGF1 (●) (1 μM each) by FVIIa (1 nM) in complex with relipidated dcTF (5 nM) was monitored in TBS/Ca2+ at room temperature. At each time point, aliquots of the activation reactions were transferred to wells of a 96-well plate containing 20 mM EDTA and the rate of FIXa generation was measured by an amidolytic activity assay as described under “Materials and methods”. B, the same as A except that the activation time course analysis was carried out by FVIIa (50 nM) in complex with soluble TF (200 nM) in solution.