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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Thromb Haemost. 2012 Sep 26;108(6):10.1160/TH12-06-0436. doi: 10.1160/TH12-06-0436

Figure 8.

Figure 8

Titration of Fl-FIXa-Cys195 and Fl-FIX-Cys195 with PCPS vesicles. A, changes in the emission intensity of fluorescein, covalently attached to Cys195 of the FIXa mutant (50 nM), were monitored upon titration with PCPS vesicles containing (◯) or lacking OR (●) as described under “Materials and methods”. B, the same as panel A except that fluorescein-labeled FIX-Cys195 zymogen was used for the titration. C, data of panels A and B are re-plotted for both fluorescein-labeled FIXa-Cys195 (□) and FIX-Cys195 (■) according to equation 1 as described under “Materials and methods”. Acceptor densities were 1.4 × 10−4 OR/Å2 for both FRET measurements.