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. 2012 Nov 13;1(11):e54. doi: 10.1038/mtna.2012.46

Figure 6.

Figure 6

The r1c3 mutant transduces HeLa cells with wt efficiency. (a) Western blot analysis shows the expression of VPs in mosaic r1c3 produced with a 1:1 molar ratio of VP1-mCherry:VP2/VP3 helper plasmids. VP2/3 capsids packaged with a GFP transgene show no expression of VP1. (b,c) HeLa cells were transduced at MOI = 2,000 and analyzed by flow cytometry after 36 hours. Transduction is visualized by epifluorescence microscopy (in c) and quantified by flow cytometry (in b). The incorporation of VP1-mCherry in r1c3-GFP completely recovers infectivity compared with wt, while VP2/3 only virions display negligible transduction. Each transduction was performed in triplicate (n = 3), with error bars indicating SD. *P value <0.001. Bar = 10 µmol/l. GFP, green fluorescent protein; MOI, multiplicity of infection.