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. 2012 Nov 27;1(11):e57. doi: 10.1038/mtna.2012.48

Figure 1.

Figure 1

Schematic depiction of the genetic and transcriptional map of representative recombinant baculoviruses carrying the toxin expression cassettes flanked by AAV ITRs. (a) DT-A gene inserted with the hGH intron and under the control of various tumor-specific promoters. (b) Barnase gene inserted with the SV40LT antigen intron and fused in-frame with the GFP gene. (c) DT-A gene without intron insertion or flanked by LoxP sites and placed in the antisense direction, and barnase-GFP gene without intron insertion. (d) Detailed sequence of the DT-A coding region (upper case letters) comprising the hGH intron (lower case letters). (e) Detailed barnase-GFP coding region (upper case letters) comprising the SV40LT antigen intron (lower case letters). AAV, adeno-associated virus; Bar, barnase; CAG, chicken β-actin promoter; CMV, cytomegalovirus promoter; DT-A, diphtheria toxin fragment A; GFP, green fluorescent protein; hAFP, human alfa-fetoprotein promoter; hCCKAR, human cholecystokinin type A receptor promoter; hCOX2, human cyclooxygenase-2 promoter; hCXCR4, human C-X-C chemokine receptor type 4 promoter; hGH, human growth hormone; hSURV, human surviving promoter; hTERT, human telomerase reverse transcriptase promoter; ITR, inverted terminal repeat; LoxP, specific sequence recognized by Cre recombinase; pA, polyadenylation signal.