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Generation of recombinant baculoviruses with or without intron insertion. (a) Generation of recombinant baculoviruses by transfecting Bacmid DNAs into Sf9 cells. (b) Amplification of recombinant baculoviruses by infecting Sf9 cells with baculovirus stocks generated from a. Bar, barnase; CAG, chicken β-actin promoter; CMV, cytomegalovirus promoter; DT-A, diphtheria toxin fragment A; GFP, green fluorescent protein; hGH, human growth hormone.
