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. 2012 Nov 27;1(11):e57. doi: 10.1038/mtna.2012.48

Figure 3.

Figure 3

The killing effects on HEK293 cells by recombinant baculoviruses harboring toxic genes. The cells were seeded in a 24-well plate (1.5 × 105 cells/well) overnight and transduced with baculoviral vectors (5 × 107 pfu/well) for 48 hours. The images were photographed with ×20 lens under (a,b,d,e) tungsten lighting or (c,f) fluorescent lighting. (a) Bac-CMV-inDTA(hGH); (b) Bac-CMV-inBar(SV40LT)-GFP; (c) same field as b; (d) untreated; (e) Bac-CMV-GFP; (f) same field as e. Bar, barnase; CMV, cytomegalovirus promoter; DT-A, diphtheria toxin fragment A; GFP, green fluorescent protein; hGH, human growth hormone; pfu, plaque-forming unit.