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(A and B) Yield of wt HSV-1(F) and R7910 in wt or sh-β3 293T cells, negative or positive for TLR2. Replicate cultures were infected (1 PFU/cell) and harvested 4 h (0 time) or 24 h after virus adsorption. The virus present in cell debris plus medium was titrated in Vero [HSV-1(F)] or U2OS (R7910) cells. Columns show the 24 h yield (PFU/mL) after subtraction of 0-time values. Results are average of three experiments ± SD.
