Fig. 1.

Anti-AC IgM with LPS stimulation inhibits MAP kinase activation and induces MKP-1. (A) Immunoblot analysis of BM-DCs incubated for 30 min in serum-free media, under the indicated conditions, with IgM at 20 μg/mL and C1q at 80 μg/mL. Whole-DC extracts were used for all replicate immunoblots. Data are representative of five or more studies. (B) Quantitative transcript analysis from BM-DCs incubated in complete media with 10% (vol/vol) FBS. Data represent means from triplicates with values normalized by GADPH content at indicated time intervals. (C) Anti-AC IgM significantly inhibits LPS-induced TGF-β activation in DCs cultured under indicated conditions for 18 h (n = 6). Error bars indicate SD. **P < 0.01.