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Anti-AC IgM requires C1q and/or MBL for induction of nuclear MKP-1 and inhibition of nuclear localized P-p38 in LPS-stimulated DCs. Immunofluorescence studies for MKP-1 and P-p38 were performed on LPS-stimulated DCs cultured in serum-free media for 30 min under the indicated conditions. Staining used mouse anti–P-p38-Alexa488, rabbit anti–MKP-1 counterstained with anti-rabbit IgG-PE, and Hoechst dye. Data are representative of six or more independent experiments.
