Fig. 2.

The P. aeruginosa H1-T6SS causes dose- and contact-dependent lysis of recipient cells. (A) TLFM image sequence of a P. aeruginosa–B. thailandensis coculture. B. thailandensis and P. aeruginosa are GFP- and mCherry-labeled, respectively. Outlined blue cells on masks lyse in subsequent frame. Arrowheads point to B. thailandensis cells that lyse following P. aeruginosa contact. Fig. S1 shows the corresponding ΔtssM1 control. (Scale bar, 6 μM.) (B and C) Lysis quantification from TLFM experiment depicted in A. Either percentage of lysis of total cellular population (B) or distinct lysis counts for indicated bacterial populations (C). Error bars depict standard counting error. N/D, none detected. (D and E) B. thailandensis (D) or S. Typhimurium (E) lysis increases linearly with increased contact to P. aeruginosa. Red dashed and solid black lines depict total cells and percentage lysis, respectively. Results represent data pooled from two independent experiments.