Fig. 3.

Tse1 acts early to promote the majority of H1-T6SS–catalyzed B. thailandensis lysis. (A) Lysis quantification from TLFM sequences of B. thailandensis coculture with the indicated P. aeruginosa strains. Error bars, ± counting error (C.E.) (B) Western blot analysis of Tse1 expression in supernatant (Sup) and cell-associated (Cell) fractions of the indicated P. aeruginosa strains. RNA polymerase (RNAP) was used as a loading control. (C) Running total of frame-by-frame B. thailandensis lysis events observed in coculture experiments with the indicated P. aeruginosa strains. Shading corresponds to C.E. (D) Neighbor-cell analysis of cocultures in C indicating similar neighbor density for each experiment. Dotted lines of corresponding color depict C.E.