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. 2012 Oct 15;109(48):19655–19660. doi: 10.1073/pnas.1209357109

Fig. 4.

Fig. 4.

Cell-based studies of selected αTAT1 mutants. (A) Representative micrographs of αTAT1−/− MEFs expressing comparable levels of GFP-αTAT1 variants. Transfected cells are outlined, GFP-αTAT1 staining is green, acetylated tubulin staining is red, and DNA is blue. Note that the untransfected αTAT1−/− MEFs display no acetylated tubulin staining. For scale, each panel is approximately 120 μm in width. (B) The ability of the αTAT1 mutants to acetylate microtubules in vivo was assessed by measuring the intensity of the acetylated tubulin immunostaining in 45 cells per mutant (for the WT, D157N, D109A, and E111A enzymes) or in 15 cells (C120A, F105A, R69A, and N182A), and normalizing the value to the intensity of the GFP-αTAT1 signal. Statistical analysis was performed by using a two-sided Wilcoxon test in R. *P < 0.05, **P < 0.01, ****P < 0.0001.