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. 2012 Nov 12;109(48):19846–19851. doi: 10.1073/pnas.1211517109

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Fig. 2. — Effects of KNDy neuron ablation on average T_SKIN in OVX (A) and OVX + E₂ rats (B). (A) Circadian rhythms of T_SKIN were not different between NK₃-SAP and Blank-SAP rats. However, OVX NK₃-SAP rats exhibited consistently lower T_SKIN than OVX Blank-SAP rats. (B) After E₂ treatment, T_SKIN was still consistently lower in NK₃-SAP rats than Blank-SAP rats. E₂ reduced T_SKIN during the dark phase in both Blank-SAP and NK₃-SAP rats (compare with A). These waveforms represent the average T_SKIN for each group (6–11 rats per group) and were generated using a moving average of five data points. Dark bars represent the dark phase of the 24-h cycle, and numbers refer to circadian recording days 3, 4, and 5. Statistical analyses of these data are shown in Fig. 3B.