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. 2012 Nov 12;109(48):19691–19696. doi: 10.1073/pnas.1210916109

Fig. 4.

Fig. 4.

ARL13B mutations that cause JBTS are not able to mediate INPP5E ciliary targeting. (A) Localization of ARL13B mutant variants. FLAG-tagged ARL13B variants (green) were transfected into IMCD3 cells and their localization was probed with anti-FLAG antibody. (B) GDP-locked ARL13B and JBTS-associated missense mutants show greatly reduced ability to bind to INPP5E. (C) ARL13B mutants fail to mediate INPP5E (green) ciliary targeting. Expression of endogenous ARL13B was ablated by siRNA targeting ARL13B 3′ UTR and indicated ARL13B variants with FLAG tags (red) were transfected. Insets show the green channel images of the boxed area. (D) Suppression of endogenous ARL13B expression by siRNA targeting ARL13B 3′ UTR used in C. β-actin was used as a loading control.