UL21 activates the UL11–UL16 interaction. Vero cells were transfected to express the indicated proteins, either alone (A), pairwise (B, D, G, H, and I), or in combinations of three (C, E, and F). At 16 to 18 h after transfection, the cells were fixed, stained with the appropriate antibodies, and examined by confocal microscopy. For double transfections, the merged images are shown in the right panels. For triple transfections, the right panels indicate which proteins were coexpressed and show the images for the two being analyzed.