Fig. 5.

gE fails to accumulate on the surface of infected Vero cells in the absence of its binding partners. (A) Vero cells were infected at an MOI of 0.01 with WT or the indicated mutant and revertant viruses. At 18 to 24 h after infection, the cells were fixed and reacted with a mouse monoclonal antibody (clone 3114) to gE before microscopy. (B) Vero cells were infected with WT or mutants at an MOI of 5. At 18 to 24 h after infection, the cells were harvested, lysed in sample buffer, subjected to electrophoresis in denaturing gels, and immunoblotted with indicated antibodies. Capsid protein VP5 was used as the loading control.