Table 1.
Comparative analysis* of gene clusters of not, not′, phq, and mal
| Not proteins (AA) | Function | Not′ proteins (AA) | Function (%identity to corresponding Not protein) | Phq proteins (AA) | Function (%identity to corresponding Not protein) | Mal proteins (AA) | Function (%identity to corresponding Not/Phq protein) |
|---|---|---|---|---|---|---|---|
| NotA (339) | Negative regulator | NotA′ (334) | Negative regulator (70% NotA) | PhqA (405) | Prenyltransferase (22% NotC) | MalA (667) | Halogenase (−/−) |
| NotB (456) | FAD monooxygenase | NotB′ (455) | FAD monooxygenase (88% NotB) | PhqB (2449) | NRPS [A-T-C-A-T-R] (26% NotE) | MalB (369) | Prenyltransferase (28% NotC/34% PhqA) |
| NotC (427) | Prenyltransferase | NotC′ (426) | Prenyltransferase (87% NotC) | PhqC (353) | 2OG-Fe(II)-oxygenase (−) | MalC (264) | Short chain dehydrogenase (−/52% PhqE) |
| NotD (621) | Oxidoreductase | NotD′ (612) | Oxidoreductase (80% NotD) | PhqD (322) | Pyrroline-5-carboxylate reductase (−) | MalD (336) | Negative regulator (36% NotA/55% PhqG) |
| NotE (2241) | NRPS [A-T-C-A-T-C] | NotE′ (2225) | NRPS [A-T-C-A-T-C] (79% NotE) | PhqE (265) | Short chain dehydrogenase (−) | MalE (438) | Prenyltransferase (36% NotF/34% PhqJ) |
| NotF (453) | Prenyltransferase | NotF′ (435) | Prenyltransferase (79% NotF) | PhqF (411) | Efflux pump (18% NotK) | MalF (590) | Oxidoreductase (37% NotD/39% PhqH) |
| NotG (544) | P450 monooxygenase | NotG′ (544) | P450 monooxygenase (87% NotG) | PhqG (338) | Negative regulator (34% NotA) | MalG (2345) | NRPS [A-T-C-A-T-R] (27% NotE/37% PhqB) |
| NotH (502) | P450 monooxygenase | NotH′ (499) | P450 monooxygenase (84% NotH) | PhqH (602) | Oxidoreductase (34% NotD) | ||
| NotI (434) | FAD monooxygenase | NotI′ (433) | FAD monooxygenase (85% NotI) | PhqI (462) | Prenyltransferase (−) | ||
| NotJ (371) | Unknown | NotJ′ (362) | Unknown (80% NotJ) | PhqJ (406) | Prenyltransferase (32% NotF) | ||
| NotK (564) | Efflux pump | NotK′ (577) | Efflux pump (14% NotK) | PhqK (459) | FAD monooxygenase (32% NotI) | ||
| NotL (484) | Transcriptional activator | NotL′ (620) | Transcriptional factor (15% NotL) | PhqL (563) | P450 monooxygenase (29% NotG) | ||
| NotM (402) | Unknown | NotM′ (454) | Unknown (−) | PhqM (536) | P450 monooxygenase (15% NotH) | ||
| NotN (340) | Dehydrogenase | NotN′ (416) | Unknown (−) | PhqN (326) | Methyltransferase | ||
| NotO (331) | Short-chain dehydrogenase | NotO′ (462) | Unknown (−) | PhqO (451) | P450 monooxygenase (−) | ||
| NotP (322) | Unknown | NotP′ (292) | Unknown (−) | ||||
| NotQ (152) | Unknown | NotQ′ (506) | Transcription factor | ||||
| NotR (461) | Transcriptional coactivator | NotR′ (172) | Unknown |
*
Genes were predicted using the FGENESH-M tool from http://www.softberry.com; Functions of gene products were predicted using BLAST search
−
Homology cannot be calculated due to unrelatedness