Figure 1. Shh Production is Increased in CD34^Shh.

A. Assessment of Shh mRNA in cells 48 hours after modification using quantitative RT-PCR (qRT-PCR). CD34^Shh show robust up-regulation of Shh mRNA as compared to CD34^NM and CD34^EV. Levels of mRNA were normalized to 18S RNA and are plotted relative to CD34^EV. Bars depicting Shh mRNA for CD34^NM and CD34^EV treatments are present but non-visible due to low expression. Data is presented as the mean ± SE where N=4. B. Assessment of Shh protein production in modified CD34+ cells using a Shh ELISA. Shh is effectively up-regulated in supernatant (SN) and CD34^Shh lysates when compared to CD34^EV. The bars indicate the absolute Shh amount derived from 1 million cells. Data are presented as the mean ± SE where N=3.