Figure 3. Reduced Infarct Size and Increased Border Zone Capillary Density in Hearts of Mice Receiving CD34^Shh.

A. Representative cross-sectional Masson-Trichrome stained histological sections depicting the infarct zone in mice receiving either saline or sub-therapeutic doses of CD34^EV or CD34^Shh. B. Graph depicting the quantification of the infarct length relative to the entire left ventricular circumference which is significantly reduced in animals receiving CD34^Shh. Bars indicate group means ± SE. C. Representative immunofluorescence images taken within the infarct border zone of mice treated with saline, CD34^EV or CD34^Shh. Capillaries were stained with BS-lectin-Alexa-555 (red) and nuclei were counterstained with DAPI (blue). D. Graph depicting the quantification of border zone capillary number across treatments presented as the number of isolectin B4 positive capillaries and DAPI stained nucleii per high power field (HPF). Bars represent group means ± SE. * represents statistical significance (p<0.05) assessed by One-Way ANOVA with the post-hoc Holm-Sidak test.