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CDR3 sequences of anti-MICA monoclones. Nucleotide and deduced amino acid sequences of V segment of (a) WW2G8, (b) WW6B7, and (c) WW9B8 compared with the reference sequence to identify CDR3. This region was randomly mutated by PCR mutagenesis creating a library of mutated CDR3 on WW2G8, WW6B7, and WW9B8 frameworks, and the high affinity phages were reselected by bio-panning. Dashes indicate identical nucleotides.
