Table 1.

Primers for PCR-random mutation of HCDR3 region of anti-MICA antibodies.

	Forward primers	Reverse primers	Size of product (bp)
(1) anti-MICA: WW2G8	5′-TAT TgT NNY NNY NNY NNY NNY  NNY NNY NNY NNY NNY NNY NNY  Tgg ggT-3′	5′-ACC CCA RNN RNN RNN RNN RNN  RNN RNN RNN RNN RNN RNN RNN  ACA ATA-3′	5000 bp
(2) anti-MICA: WW6B7	5′-TAC TgT NNY NNY NNY NNY NNY  NNY NNY NNY NNY NNY NNY NNY  NNY  NNY Tgg ggC-3′	5′- gCC CCA RNN RNN RNN RNN RNN  RNN RNN RNN RNN RNN RNN RNN  RNN RNN ACA gTA-3′	5000 bp
(3) anti-MICA: WW9B8	5′-TAT TgT NNY NNY NNY NNY NNY  NNY NNY NNY NNY NNY NNY NNY  Tgg ggT-3′	5′-ACC CCA RNN RNN RNN RNN RNN  RNN RNN RNN RNN RNN RNN RNN  ACA ATA-3′	5000 bp