Figure 6.

Eight-month-old NF1^Nestin mice show enhanced basal proliferation and neurogenesis. A, B, Immunostaining for Ki67 (green) and Dcx (red) revealed a higher level of proliferation and neurogenesis in the DG of NF1^Nestin mice compared with control mice. NeuN, blue. Scale bar, 50 μm. C, D, Quantitative analyses of the dendritic morphology of Dcx+ immature neurons showed that NF1^Nestin mice had an increased number of dendritic intersections (C) and longer length (D) compared with controls. E, Diagram of BrdU pulse-chase analysis. Mice were induced with tamoxifen at 2 months of age and injected with BrdU at 8 months of age to label cells that were born at 8 months and were present 28 days later. Results are mean + SEM. *p < 0.05, **p < 0.01, ***p < 0.001.