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. Author manuscript; available in PMC: 2012 Dec 3.
Published in final edited form as: Circulation. 2011 Jul 18;124(5):10.1161/CIRCULATIONAHA.110.005108. doi: 10.1161/CIRCULATIONAHA.110.005108

Figure 3. Shear stress-induction of KLF2 is mediated through miR-92a.

Figure 3

(A) HUVECs were exposed to laminar flow for 4, 8 or 16 hr. qRT-PCR was performed to detect the level of miR-92a, which was normalized to that of U6 RNA. * p<0.05 compared with static controls (time 0), analyzed by one-way ANOVA followed by Dunnett’s test. (B–F) HUVECs were transfected with 20 nM control RNA or pre-92a for 48 hr and then exposed to laminar flow for 8 hr. (B) KLF2 mRNA level was detected by qRT-PCR and (C) protein level was assessed by Western blot analysis. (D,E) eNOS and TM mRNA levels were detected by qRT-PCR and (F) protein level was assessed by Western blot analysis, and the results of statistical analyses are shown in the right. The data represent mean±SD from 3 independent experiments. * p<0.05 between the indicated groups, analyzed by two-way ANOVA followed by the Bonferroni posthoc test.