RNA expression of FDH and SHMT after spraying
leaves with various metabolites. Each lane was loaded with 10 μg of
RNA. Ctr Lf, Control leaves (water); For 2h, For 8h, and For 24h, 2, 8,
and 24 h after 10 mm formate application,
respectively; ABA 2h, ABA 8h, and ABA 24h, 2, 8, and 24 h after
spraying 100 μm ABA, respectively; Ser 24h, 24 h
after 10 mm Ser application; Sar 24h, 24 h after
spraying 10 mm sarcosine; Ac 24h, 24 h after spraying
10 mm acetate; Pyr 24h, 24 h after spraying 10
mm pyruvic acid; Et 2h and Et 24h, 2 and 24 h after
spraying 10% ethanol, respectively; and Me 24h, 24 h after
spraying 20% methanol. The autoradiographs were scanned using the
control leaf sample as the reference for both probes, and the figures
were corrected according to the RNA loadings.