Fig. 3.

Number of NKB-ir neurons in the ARC of male and female rats after neonatal estrogenization. NKB-ir cells were counted in the ARC of adult male and female rats, treated neonatally with vehicle (controls) or an effective dose of EB. Representative photomicrographs of IHC assays are shown for the four groups, at ×40 magnification. Quantitative values of NKB-ir neurons within the ARC in the corresponding experimental groups are provided in the histogram. In addition, a schematic drawing depicting the area of counting of NKB-ir cells has been included. Groups with different superscript letters are statistically different (ANOVA followed by Student-Newman-Keuls multiple range test). Bar, 50 μm. VEH, Vehicle.